NEDD4 Mouse Monoclonal Antibody [Clone ID: OTI6E12]

CAT#: CF812646

Carrier-free (BSA/glycerol-free) NEDD4 mouse monoclonal antibody,clone OTI6E12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEDD4 (RC212738, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEDD4 (1:500). Positive lysates LY405051 (100ug) and LC405051 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6E12
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 160-460 of human NEDD4 (NP_940682) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 140.7 kDa
• Gene Name: Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 2, mRNA.
• Database Link:
  NP_940682
  Entrez Gene 4734 Human
  UniProt ID P46934
• Background: This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016]
• Synonyms: NEDD4-1; RPF1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Ubiquitin mediated proteolysis