Complement Component 6 (C6) Mouse Monoclonal Antibody [Clone ID: OTI1H4]

CAT#: CF812708

Carrier-free (BSA/glycerol-free) C6 mouse monoclonal antibody,clone OTI1H4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C6 (RC207784, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C6 (1:500). Positive lysates LY400016 (100ug) and LC400016 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts from human plasma by using anti-C6 monoclonal antibody (1:250).

Specifications

Product Data

• Clone Name: OTI1H4
• Applications: WB
• Recommended Dilution: WB 1:250~500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 176-522 of human C6 (NP_000056) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 104.9 kDa
• Gene Name: Homo sapiens complement C6 (C6), transcript variant 1, mRNA.
• Database Link:
  NP_000056
  Entrez Gene 729 Human
  UniProt ID P13671
• Background: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. [UniProtKB/Swiss-Prot Function]

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus