C1orf19 (TSEN15) Mouse Monoclonal Antibody [Clone ID: OTI3F5]

CAT#: CF812720

Carrier-free (BSA/glycerol-free) TSEN15 mouse monoclonal antibody,clone OTI3F5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSEN15 (RC205519, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSEN15 (1:500). Positive lysates LY409366 (100ug) and LC409366 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-TSEN15 monoclonal antibody (1:250).

Specifications

Product Data

• Clone Name: OTI3F5
• Applications: WB
• Recommended Dilution: WB 1:250~500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TSEN15 (NP_443197) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.5 kDa
• Gene Name: Homo sapiens tRNA splicing endonuclease subunit 15 (TSEN15), transcript variant 1, mRNA.
• Database Link:
  NP_443197
  Entrez Gene 289083 RatEntrez Gene 116461 Human
  UniProt ID Q8WW01
• Background: This gene encodes a subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from tRNA precursors. Alternative splicing results in multiple transcript variants. There is a pseudogene of this gene on chromosome 17. [provided by RefSeq, Jul 2014]
• Synonyms: C1orf19; PCH2F; sen15