M6PR Mouse Monoclonal Antibody [Clone ID: OTI2F3]

CAT#: CF812721

Carrier-free (BSA/glycerol-free) M6PR mouse monoclonal antibody,clone OTI2F3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY M6PR (RC201277, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-M6PR (1:500). Positive lysates LY419372 (100ug) and LC419372 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 13 different cell lines by using anti-M6PR monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI2F3
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 27-185 of human M6PR (NP_002346) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.99 kDa
• Gene Name: Homo sapiens mannose-6-phosphate receptor, cation dependent (M6PR), transcript variant 1, mRNA.
• Database Link:
  NP_002346
  Entrez Gene 17113 MouseEntrez Gene 312689 RatEntrez Gene 477700 DogEntrez Gene 716696 MonkeyEntrez Gene 4074 Human
  UniProt ID P20645
• Background: Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. [UniProtKB/Swiss-Prot Function]
• Synonyms: CD-M6PR; CD-MPR; MPR-46; MPR 46; MPR46; SMPR

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome