ARSG Mouse Monoclonal Antibody [Clone ID: OTI2A8]

CAT#: CF812731

Carrier-free (BSA/glycerol-free) ARSG mouse monoclonal antibody,clone OTI2A8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARSG (RC201967, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARSG (1:500). Positive lysates LY402396 (100ug) and LC402396 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2A8
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 17-525 of human ARSG (NP_055775) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.9 kDa
• Gene Name: Homo sapiens arylsulfatase G (ARSG), transcript variant 1, mRNA.
• Database Link:
  NP_055775
  Entrez Gene 22901 Human
  UniProt ID Q96EG1
• Background: The protein encoded by this gene belongs to the sulfatase enzyme family. Sulfatases hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules. This protein displays arylsulfatase activity at acidic pH, as is typical of lysosomal sulfatases, and has been shown to localize in the lysosomes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2012]
• Synonyms: USH4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysosome