EFHD1 Mouse Monoclonal Antibody [Clone ID: OTI8F3]

CAT#: CF812755

Carrier-free (BSA/glycerol-free) EFHD1 mouse monoclonal antibody,clone OTI8F3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EFHD1 (RC200956, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EFHD1 (1:2000). Positive lysates LY403060 (100ug) and LC403060 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 1 cell lines lysate by using anti-EFHD1 monoclonal antibody (1:250).

Specifications

Product Data

• Clone Name: OTI8F3
• Applications: WB
• Recommended Dilution: WB 1:250~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EFHD1 (NP_079478) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.7 kDa
• Gene Name: Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 1, mRNA.
• Database Link:
  NP_079478
  Entrez Gene 80303 Human
  UniProt ID Q9BUP0
• Background: This gene encodes a member of the EF-hand super family of calcium binding proteins, which are involved in a variety of cellular processes including mitosis, synaptic transmission, and cytoskeletal rearrangement. The protein encoded by this gene is composed of an N-terminal disordered region, proline-rich elements, two EF-hands, and a C-terminal coiled-coil domain. This protein has been shown to associate with the mitochondrial inner membrane, and in HeLa cells, acts as a novel mitochondrial calcium ion sensor for mitochondrial flash activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]
• Synonyms: MST133; MSTP133; PP3051; SWS2