PSMA1 Mouse Monoclonal Antibody [Clone ID: OTI9H1]

CAT#: CF812757

Carrier-free (BSA/glycerol-free) PSMA1 mouse monoclonal antibody,clone OTI9H1

Product Images

Western blot analysis of extracts (35ug) from 8 cell lines and 2 tissue lysates by using anti-PSMA1 monoclonal antibody (1:250).

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  Immunofluorescent staining of Hela cells using anti-PSMA1 antibody (TA812757/green, upper left; DAPI/blue, lower left; MERGED, upper right) or Isotype control (MERGED, lower right) (1:100).

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  Immunofluorescent staining of HEK293T cells using anti-PSMA1 antibody (TA812757/green, upper left; DAPI/blue, lower left; MERGED, upper right) or Isotype control (MERGED, lower right) (1:100).

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  Immunofluorescent staining of NCI-H460 cells using anti-PSMA1 antibody (TA812757/green, upper left; DAPI/blue, lower left; MERGED, upper right) or Isotype control (MERGED, lower right) (1:100).

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  Immunofluorescent staining of PC3 cells using anti-PSMA1 antibody (TA812757/green, upper left; DAPI/blue, lower left; MERGED, upper right) or Isotype control (MERGED, lower right) (1:100).

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Specifications

Product Data

• Clone Name: OTI9H1
• Applications: IF, WB
• Recommended Dilution: WB 1:250, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PSMA1 (NP_002777) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.4 kDa
• Gene Name: Homo sapiens proteasome subunit alpha 1 (PSMA1), transcript variant 2, mRNA.
• Database Link:
  NP_002777
  Entrez Gene 26440 MouseEntrez Gene 29668 RatEntrez Gene 5682 Human
  UniProt ID P25786
• Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2009]
• Synonyms: HC2; HEL-S-275; NU; PROS30

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome