Aspartyl Aminopeptidase (DNPEP) Mouse Monoclonal Antibody [Clone ID: OTI8H3]

CAT#: CF812760

Carrier-free (BSA/glycerol-free) DNPEP mouse monoclonal antibody,clone OTI8H3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNPEP (RC200104, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNPEP (1:500). Positive lysates LY402151 (100ug) and LC402151 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 11 different cell lines by using anti-DNPEP monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI8H3
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DNPEP (NP_036232) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.2 kDa
• Gene Name: Homo sapiens aspartyl aminopeptidase (DNPEP), transcript variant 3, mRNA.
• Database Link:
  NP_036232
  Entrez Gene 13437 MouseEntrez Gene 301529 RatEntrez Gene 478922 DogEntrez Gene 703180 MonkeyEntrez Gene 23549 Human
  UniProt ID Q9ULA0
• Background: The protein encoded by this gene is an aminopeptidase which prefers acidic amino acids, and specifically favors aspartic acid over glutamic acid. It is thought to be a cytosolic protein involved in general metabolism of intracellular proteins. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
• Synonyms: ASPEP; DAP

Reference Data

• Protein Families: Druggable Genome, Protease