ARHGEF4 Mouse Monoclonal Antibody [Clone ID: OTI1A6]

CAT#: CF812762

Carrier-free (BSA/glycerol-free) ARHGEF4 mouse monoclonal antibody, clone OTI1A6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGEF4 (RC215591, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGEF4.(1:500)

Specifications

Product Data

• Clone Name: OTI1A6
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-363 of human ARHGEF4 (NP_056135) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78.9 kDa
• Gene Name: Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 1, mRNA.
• Database Link:
  NP_056135
  Entrez Gene 50649 Human
  UniProt ID Q9NR80
• Background: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013].
• Synonyms: ASEF; ASEF1; GEF4; SMIM39; STM6

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton