TAGLN2 Mouse Monoclonal Antibody [Clone ID: OTI7A5]

CAT#: CF812766

Carrier-free (BSA/glycerol-free) TAGLN2 mouse monoclonal antibody,clone OTI7A5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TAGLN2 (RC203473, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAGLN2 (1:500). Positive lysates LY418584 (100ug) and LC418584 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-TAGLN2 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI7A5
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TAGLN2 (NP_003555) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.2 kDa
• Gene Name: Homo sapiens transgelin 2 (TAGLN2), transcript variant 2, mRNA.
• Database Link:
  NP_003555
  Entrez Gene 21346 MouseEntrez Gene 304983 RatEntrez Gene 8407 Human
  UniProt ID P37802
• Background: The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The specific function of this protein has not yet been determined, although it is thought to be a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]
• Synonyms: HA1756