Lysyl tRNA synthetase (KARS) Mouse Monoclonal Antibody [Clone ID: OTI1E7]

CAT#: CF812796

Carrier-free (BSA/glycerol-free) KARS mouse monoclonal antibody,clone OTI1E7

Product Images

Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-KARS monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-KARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812796) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-KARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812796) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-KARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812796) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812796) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812796) (1:500)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-KARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812796) (1:500)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-KARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812796) (1:500)

• Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-KARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812796) (1:500)

Show all 9 images

Specifications

Product Data

• Clone Name: OTI1E7
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human KARS (NP_005539) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67.9 kDa
• Gene Name: Homo sapiens lysyl-tRNA synthetase (KARS), transcript variant 2, mRNA.
• Database Link:
  NP_005539
  Entrez Gene 85305 MouseEntrez Gene 292028 RatEntrez Gene 3735 Human
  UniProt ID Q15046
• Background: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: CMTRIB; DFNB89; KARS1; KARS2; KRS

Reference Data

• Protein Pathways: Aminoacyl-tRNA biosynthesis