Rab5 (RAB5A) Mouse Monoclonal Antibody [Clone ID: OTI6D9]

CAT#: CF812808

Carrier-free (BSA/glycerol-free) RAB5A mouse monoclonal antibody,clone OTI6D9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB5A (RC203873, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAB5A (1:500).

Specifications

Product Data

• Clone Name: OTI6D9
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RAB5A (NP_004153) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.5 kDa
• Gene Name: Homo sapiens RAB5A, member RAS oncogene family (RAB5A), transcript variant 1, mRNA.
• Database Link:
  NP_004153
  Entrez Gene 64633 RatEntrez Gene 271457 MouseEntrez Gene 5868 Human
  UniProt ID P20339
• Synonyms: RAB5

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Endocytosis