MSF (SEPT9) Mouse Monoclonal Antibody [Clone ID: OTI3G2]

CAT#: CF812811

Carrier-free (BSA/glycerol-free) SEPT9 mouse monoclonal antibody,clone OTI3G2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEPT9 (RC200264, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEPT9 (1:2000).

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  Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-SEPT9 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI3G2
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SEPT9 (NP_006631) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.5 kDa
• Gene Name: Homo sapiens septin 9 (SEPTIN9), transcript variant 3, mRNA.
• Database Link:
  NP_006631
  Entrez Gene 10801 Human
  UniProt ID Q9UHD8
• Background: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2009]
• Synonyms: AF17q25; MSF; MSF1; NAPB; PNUTL4; SEPT9; SeptD1; SINT1

Reference Data

• Protein Families: Druggable Genome