JAK3 Mouse Monoclonal Antibody [Clone ID: OTI1B4]

CAT#: CF812815

Carrier-free (BSA/glycerol-free) JAK3 mouse monoclonal antibody,clone OTI1B4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JAK3 (RC217928, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JAK3. Positive lysates LY424859 (100ug) and LC424859 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1B4
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 811-1124 of human JAK3 (NP_000206) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 124.9 kDa
• Gene Name: Homo sapiens Janus kinase 3 (JAK3), mRNA.
• Database Link:
  NP_000206
  Entrez Gene 3718 Human
  UniProt ID P52333
• Background: The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]
• Synonyms: JAK-3; JAK3_HUMAN; JAKL; L-JAK; LJAK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Jak-STAT signaling pathway, Primary immunodeficiency