Syntaxin 1a (STX1A) Mouse Monoclonal Antibody [Clone ID: OTI2D11]

CAT#: CF812826

Carrier-free (BSA/glycerol-free) STX1A mouse monoclonal antibody,clone OTI2D11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STX1A (RC209062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STX1A (1:500).

• 

  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-STX1A monoclonal antibody (1:500).

• 

  Western blot analysis of extracts (35ug) from 2 different tissue lysates by using anti-STX1A monoclonal antibody (1:500).

• 

  Immunofluorescent staining of SF-268 cells using anti-STX1A antibody (TA812826/green, upper left; DAPI/blue, lower left; MERGED, upper right) or Isotype control (MERGED, lower right) (1:100).

Specifications

Product Data

• Clone Name: OTI2D11
• Applications: IF, WB
• Recommended Dilution: WB 1:500, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human STX1A (NP_004594) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens syntaxin 1A (STX1A), transcript variant 1, mRNA.
• Database Link:
  NP_004594
  Entrez Gene 20907 MouseEntrez Gene 116470 RatEntrez Gene 6804 Human
  UniProt ID Q16623
• Background: This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
• Synonyms: HPC-1; P35-1; STX1; SYN1A

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport