Cathepsin L (CTSL) Mouse Monoclonal Antibody [Clone ID: OTI5A5]

CAT#: CF812845

Carrier-free (BSA/glycerol-free) CTSL mouse monoclonal antibody,clone OTI5A5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTSL (RC203143, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTSL (1:500). Positive lysates LY400711 (100ug) and LC400711 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 cell lines lysates by using anti-CTSL monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-CTSL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812845) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CTSL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812845) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CTSL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812845) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-CTSL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812845) (1:500)

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Specifications

Product Data

• Clone Name: OTI5A5
• Applications: IHC, WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 114-333 of human CTSL (NP_001903) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.53 kDa
• Gene Name: Homo sapiens cathepsin L (CTSL), transcript variant 1, mRNA.
• Database Link:
  NP_001903
  Entrez Gene 1514 Human
  UniProt ID P07711
• Background: The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]
• Synonyms: CATL; CTSL1; MEP

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Antigen processing and presentation, Lysosome