NG2 (CSPG4) Mouse Monoclonal Antibody [Clone ID: OTI3C12]

CAT#: CF812900

Carrier-free (BSA/glycerol-free) CSPG4 mouse monoclonal antibody,clone OTI3C12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSPG4 (RC218462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSPG4 (1:500).

Specifications

Product Data

• Clone Name: OTI3C12
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1950-2210 of human CSPG4 (NP_001888) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 250.4 kDa
• Gene Name: Homo sapiens chondroitin sulfate proteoglycan 4 (CSPG4), mRNA.
• Database Link:
  NP_001888
  Entrez Gene 1464 Human
  UniProt ID Q6UVK1
• Background: A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]
• Synonyms: HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2

Reference Data

• Protein Families: Transmembrane