Cytoglobin (CYGB) Mouse Monoclonal Antibody [Clone ID: OTI5G1]

CAT#: CF812909

Carrier-free (BSA/glycerol-free) CYGB mouse monoclonal antibody,clone OTI5G1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYGB (RC206642, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYGB (1:500).

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  Western blot analysis of extracts (35ug) from 1 tissue lysates by using anti-CYGB monoclonal antibody (1:250).

Specifications

Product Data

• Clone Name: OTI5G1
• Applications: WB
• Recommended Dilution: WB 1:250~500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CYGB (NP_599030) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.2 kDa
• Gene Name: Homo sapiens cytoglobin (CYGB), mRNA.
• Database Link:
  NP_599030
  Entrez Gene 114757 Human
  UniProt ID Q8WWM9
• Background: This gene encodes a globin protein found in vertebrate cells. The encoded protein is described as a hexacoordinate hemoglobin which binds ligand differently from the pentacoordinate hemoglobins involved in oxygen transport, and may be involved in protection during oxidative stress. This gene is located on chromosome 17 in the same region as a retinal gene which is mutated in progressive rod-cone degeneration, but in the opposite orientation. [provided by RefSeq, Jan 2012]
• Synonyms: HGB; STAP