TIGIT Mouse Monoclonal Antibody [Clone ID: OTI8G7]

CAT#: CF812978

Carrier-free (BSA/glycerol-free) TIGIT mouse monoclonal antibody, clone OTI8G7

Product Images

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TIGIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA812978)

Specifications

Product Data

• Clone Name: OTI8G7
• Applications: IHC
• Recommended Dilution: IHC 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TIGIT (NP_776160) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.3 kDa
• Gene Name: Homo sapiens T cell immunoreceptor with Ig and ITIM domains (TIGIT), mRNA.
• Database Link:
  NP_776160
  Entrez Gene 201633 Human
  UniProt ID Q495A1
• Background: This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. [provided by RefSeq, Sep 2009]
• Synonyms: VSIG9; VSTM3; WUCAM

Reference Data

• Protein Families: Transmembrane