p75 NGF Receptor (NGFR) Mouse Monoclonal Antibody [Clone ID: OTI8A7]

CAT#: CF812986

Carrier-free (BSA/glycerol-free) NGFR mouse monoclonal antibody,clone OTI8A7

Product Images

Flow cytometric analysis of living 293T cells transfected with NGFR overexpression plasmid (RC207966), Red)/empty vector (PS100001, Blue) using anti-NGFR antibody (TA812986). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI8A7
• Applications: FC
• Recommended Dilution: FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human Ngfr (NP_002498) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.18 kDa
• Gene Name: Homo sapiens nerve growth factor receptor (NGFR), mRNA.
• Database Link:
  NP_002498
  Entrez Gene 18053 MouseEntrez Gene 24596 RatEntrez Gene 4804 Human
  UniProt ID P08138
• Background: Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]
• Synonyms: CD271; Gp80-LNGFR; p75(NTR); p75NTR; TNFRSF16

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Neurotrophin signaling pathway