VRL1 (TRPV2) Mouse Monoclonal Antibody [Clone ID: OTI2G10]

CAT#: CF813002

Carrier-free (BSA/glycerol-free) TRPV2 mouse monoclonal antibody,clone OTI2G10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRPV2 (RC202821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPV2 (1:500).

Specifications

Product Data

• Clone Name: OTI2G10
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 655-764 of human TRPV2 (NP_057197) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.8 kDa
• Gene Name: Homo sapiens transient receptor potential cation channel subfamily V member 2 (TRPV2), mRNA.
• Database Link:
  NP_057197
  Entrez Gene 51393 Human
  UniProt ID Q9Y5S1
• Background: This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. [provided by RefSeq, Jul 2008]
• Synonyms: VRL; VRL-1; VRL1

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane