PD L1 (CD274) Human Monoclonal Antibody [Clone ID: OTI8C8]

CAT#: CF813015

Carrier-free (BSA/glycerol-free) A humanized monoclonal antibody against PDL1 expressed in CHO cells according to Avelumab sequence

Product Images

Flow cytometric Analysis of living HCC78 cells, using anti-PDL1 antibody (TA813015, Avelumab, red), compared to an IgG isotype control (blue) (1:100).

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  Flow cytometric analysis of living PDL1/CD274 stable expression cells using anti-CD274 antibody (TA813015, Avelumab) (Red) compared to an IgG isotype control antibody (Blue) (1:100).

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  Detection of PDL1 neutralizing antibody using MACS column. GFP+/PDL1+ 293T cells (co-transfected with PDL1 and GFP plasmid (RC213071, PS10010) were incubated with either PDL1 antibody TA813015, Avelumab (right), negative control antibody (middle) or PBS (left) and then mixed with PD1+ 293T cells (RC210364) linked with magnetic-beads. The mixed cells were pulled down using MACS column (Miltenyi Biotec) and analysed by Flow Cytometry. GFP+/PDL1+ cells would not be collected if PD1/PDL1 interaction is neutralized by the tested antibody (1:50).

Specifications

Product Data

• Clone Name: OTI8C8
• Applications: FC
• Recommended Dilution: FLOW 1:50~100
• Reactivities: Human
• Host: Human
• Isotype: IgG1
• Clonality: Monoclonal
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.28 kDa
• Gene Name: Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.
• Database Link:
  NP_054862
  Entrez Gene 29126 Human
  UniProt ID Q9NZQ7
• Background: This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
• Synonyms: B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1; PDCD1LG1; PDL1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)