Major Basic Protein (PRG2) Mouse Monoclonal Antibody [Clone ID: OTI1C12]

CAT#: CF813063

Carrier-free (BSA/glycerol-free) PRG2 mouse monoclonal antibody,clone OTI1C12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRG2 (RC203256, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRG2 (1:500).

Specifications

Product Data

• Clone Name: OTI1C12
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 17-222 of human PRG2 (NP_002719) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.23 kDa
• Gene Name: Homo sapiens proteoglycan 2, pro eosinophil major basic protein (PRG2), transcript variant 1, mRNA.
• Database Link:
  NP_002719
  Entrez Gene 5553 Human
  UniProt ID P13727
• Background: The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. The encoded protein contains a peptide that displays potent antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, and fungi. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
• Synonyms: BMPG; MBP; MBP1; proMBP

Reference Data

• Protein Families: Secreted Protein
• Protein Pathways: Asthma