Diazepam Binding Inhibitor (DBI) Mouse Monoclonal Antibody [Clone ID: OTI6E12]

CAT#: CF813070

Carrier-free (BSA/glycerol-free) DBI mouse monoclonal antibody,clone OTI6E12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBI (RC208982, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBI (1:500).

Specifications

Product Data

• Clone Name: OTI6E12
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-104 of human DBI (NP_065438) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 11.6 kDa
• Gene Name: Homo sapiens diazepam binding inhibitor, acyl-CoA binding protein (DBI), transcript variant 1, mRNA.
• Database Link:
  NP_065438
  Entrez Gene 1622 Human
  UniProt ID P07108
• Background: Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor. [UniProtKB/Swiss-Prot Function]
• Synonyms: ACBD1; ACBP; CCK-RP; EP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: PPAR signaling pathway