MTRF1 Mouse Monoclonal Antibody [Clone ID: OTI5D6]

CAT#: CF813119

Carrier-free (BSA/glycerol-free) MTRF1 mouse monoclonal antibody,clone OTI5D6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTRF1 (RC207912, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTRF1 (1:500).

Specifications

Product Data

• Clone Name: OTI5D6
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 240-445 of human MTRF1 (NP_004285) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.1 kDa
• Gene Name: Homo sapiens mitochondrial translation release factor 1 (MTRF1), transcript variant 5, mRNA.
• Database Link:
  NP_004285
  Entrez Gene 9617 Human
  UniProt ID O75570
• Background: Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA. [UniProtKB/Swiss-Prot Function]
• Synonyms: MRF1; MTTRF1; RF1