AMPK beta 1 (PRKAB1) Mouse Monoclonal Antibody [Clone ID: OTI8D5]

CAT#: CF813121

Carrier-free (BSA/glycerol-free) PRKAB1 mouse monoclonal antibody,clone OTI8D5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAB1 (RC203911, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAB1 (1:500).

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  Western blot analysis of extracts (35ug) from 10 cell lines lysates by using anti-PRKAB1 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI8D5
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-270 of human PRKAB1 (NP_006244) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.2 kDa
• Gene Name: Homo sapiens protein kinase AMP-activated non-catalytic subunit beta 1 (PRKAB1), mRNA.
• Database Link:
  NP_006244
  Entrez Gene 19079 MouseEntrez Gene 83803 RatEntrez Gene 695737 MonkeyEntrez Gene 5564 Human
  UniProt ID Q9Y478
• Background: Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). [UniProtKB/Swiss-Prot Function]
• Synonyms: AMPK; HAMPKb

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway