B7-2 (CD86) Mouse Monoclonal Antibody [Clone ID: OTI8C9]

CAT#: CF813131

Carrier-free (BSA/glycerol-free) CD86 mouse monoclonal antibody,clone OTI8C9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD86 (RC217341, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD86 (1:1000).

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CD86 monoclonal antibody (1:500).

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  Flow cytometric analysis of living 293T cells transfected with CD86 overexpression plasmid (RC217341), Red) using anti-CD86 antibody (TA813131). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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  Flow cytometric analysis of living Raji cells, using anti-CD86 antibody (TA813131, Red), compared to an isotype control (green) (1:100).

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  Flow cytometric analysis of living Ramos cells, using anti-CD86 antibody (TA813131, Red), compared to an isotype control (green) (1:100).

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Specifications

Product Data

• Clone Name: OTI8C9
• Applications: FC, WB
• Recommended Dilution: WB 1:500~1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD86 (NP_787058) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.1 kDa
• Gene Name: Homo sapiens CD86 molecule (CD86), transcript variant 1, mRNA.
• Database Link:
  NP_787058
  Entrez Gene 942 Human
  UniProt ID P42081
• Background: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, May 2011]
• Synonyms: B7-2; B7.2; B70; CD28LG2; LAB72

Reference Data

• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis