TRPS1 Mouse Monoclonal Antibody [Clone ID: OTI9E7]

CAT#: CF813181

Carrier-free (BSA/glycerol-free) TRPS1 mouse monoclonal antibody,clone OTI9E7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRPS1 (RC215856, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPS1 (1:500).

Specifications

Product Data

• Clone Name: OTI9E7
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 985-1281 of human TRPS1 (NP_054831) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 142.8 kDa
• Gene Name: Homo sapiens transcriptional repressor GATA binding 1 (TRPS1), transcript variant 1, mRNA.
• Database Link:
  NP_054831
  Entrez Gene 83925 MouseEntrez Gene 7227 Human
  UniProt ID Q9UHF7
• Background: This gene encodes a transcription factor that represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcriptional activity. Defects in this gene are a cause of tricho-rhino-phalangeal syndrome (TRPS) types I-III. [provided by RefSeq, Jul 2008]
• Synonyms: GC79; LGCR

Reference Data

• Protein Families: Druggable Genome, Transcription Factors