CD70 Mouse Monoclonal Antibody [Clone ID: OTI5E9]

CAT#: CF813206

Carrier-free (BSA/glycerol-free) CD70 mouse monoclonal antibody,clone OTI5E9

Product Images

Flow cytometric analysis of living 293T cells transfected with CD70 overexpression plasmid (RC200410), Red)/empty vector (PS100001, Blue) using anti-CD70 antibody (TA813206). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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  Flow cytometric analysis of living Raji cells, using anti-CD70 antibody (TA813206, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

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  Flow cytometric analysis of living 786-O cells, using anti-CD70 antibody (TA813206, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-CD70 antibody (TA813206) (1:100).

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h using anti-CD70 antibody (TA813206) (Right). Cells incubated with a non-specific antibody (Left) were used as isotype control (1:100).

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Specifications

Product Data

• Clone Name: OTI5E9
• Applications: FC
• Recommended Dilution: FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD70 (NP_001243) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.9 kDa
• Gene Name: Homo sapiens CD70 molecule (CD70), transcript variant 1, mRNA.
• Database Link:
  NP_001243
  Entrez Gene 970 Human
  UniProt ID P32970
• Background: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. [UniProtKB/Swiss-Prot Function]
• Synonyms: CD27-L; CD27L; CD27LG; LPFS3; TNFSF7; TNLG8A

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction