ICOS Mouse Monoclonal Antibody [Clone ID: OTI9D2]

CAT#: CF813219

Carrier-free (BSA/glycerol-free) ICOS mouse monoclonal antibody,clone OTI9D2

Product Images

Flow cytometric analysis of living 293T cells transfected with ICOS overexpression plasmid (RC207031), Red)/empty vector (PS100001, Blue) using anti-ICOS antibody (TA813219). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:400).

• 

  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-ICOS antibody (TA813219) (1:100).

• 

  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h using anti-ICOS antibody (TA813219) (Right). Cells incubated with a non-specific antibody (Left) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI9D2
• Applications: FC
• Recommended Dilution: FLOW 1:100~400
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ICOS (NP_036224) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.2 kDa
• Gene Name: Homo sapiens inducible T cell costimulator (ICOS), mRNA.
• Database Link:
  NP_036224
  Entrez Gene 29851 Human
  UniProt ID Q9Y6W8
• Background: The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]
• Synonyms: AILIM; CD278; CVID1

Reference Data

• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Primary immunodeficiency, T cell receptor signaling pathway