B7H3 (CD276) Mouse Monoclonal Antibody [Clone ID: OTI1A10]

CAT#: CF813302

Carrier-free (BSA/glycerol-free) CD276 mouse monoclonal antibody, clone OTI1A10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD276 (RC215291, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD276.(1:1000)

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CD276 monoclonal antibody. (1:500)

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CD276 monoclonal antibody. (1:500)

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  Flow cytometric analysis of living 293T cells transfected with CD276 overexpression plasmid (RC215291), Red)/empty vector (PS100001, Blue) using anti-CD276 antibody (TA813302). Cells incubated with a non-specific antibody (Green) were used as isotype control.（1：100）

Specifications

Product Data

• Clone Name: OTI1A10
• Applications: FC, WB
• Recommended Dilution: WB 1:500-1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD276 (NP_079516) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.6 kDa
• Gene Name: Homo sapiens CD276 molecule (CD276), transcript variant 2, mRNA.
• Database Link:
  NP_079516
  Entrez Gene 102657 MouseEntrez Gene 315716 RatEntrez Gene 80381 Human
  UniProt ID Q5ZPR3
• Background: The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].
• Synonyms: 4Ig-B7-H3; B7-H3; B7H3; B7RP-2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)