MYL9 Mouse Monoclonal Antibody [Clone ID: OTI3H6]

CAT#: CF813394

Carrier-free (BSA/glycerol-free) MYL9 mouse monoclonal antibody,clone OTI3H6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYL9 (RC211412, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYL9 (1:500).

Specifications

Product Data

• Clone Name: OTI3H6
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MYL9 (NP_006088) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19.6 kDa
• Gene Name: Homo sapiens myosin light chain 9 (MYL9), transcript variant 1, mRNA.
• Database Link:
  NP_006088
  Entrez Gene 98932 MouseEntrez Gene 296313 RatEntrez Gene 10398 Human
  UniProt ID P24844
• Background: Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: LC20; MLC-2C; MLC2; MRLC1; MYRL2

Reference Data

• Protein Pathways: Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction, Vascular smooth muscle contraction