Nephrin (NPHS1) Mouse Monoclonal Antibody [Clone ID: OTI9D3]

CAT#: CF813414

Carrier-free (BSA/glycerol-free) NPHS1 mouse monoclonal antibody, clone OTI9D3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPHS1 (RC217677, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPHS1.(1:1000)

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  Western blot analysis of extracts (35ug) from 1 tissue lysate by using anti-NPHS1 monoclonal antibody. (1:500)

Specifications

Product Data

• Clone Name: OTI9D3
• Applications: WB
• Recommended Dilution: WB 1:500-1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1077-1241 of human NPHS1 (NP_004637) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 134.6 kDa
• Gene Name: Homo sapiens NPHS1 adhesion molecule, nephrin (NPHS1), mRNA.
• Database Link:
  NP_004637
  Entrez Gene 4868 Human
  UniProt ID O60500
• Background: This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]
• Synonyms: CNF; nephrin; NPHN

Reference Data

• Protein Families: Druggable Genome, Transmembrane