Nephrin (NPHS1) Mouse Monoclonal Antibody [Clone ID: OTI9D3]

CAT#: CF813414

Carrier-free (BSA/glycerol-free) NPHS1 mouse monoclonal antibody, clone OTI9D3

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USD 465.00

4 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI9D3
Applications WB
Recommended Dilution WB 1:500-1000
Reactivities Human
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 1077-1241 of human NPHS1 (NP_004637) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 134.6 kDa
Gene Name Homo sapiens NPHS1 adhesion molecule, nephrin (NPHS1), mRNA.
Background This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]
Synonyms CNF; nephrin; NPHN
Reference Data
Protein Families Druggable Genome, Transmembrane

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.