MICB Mouse Monoclonal Antibody [Clone ID: OTI4E9]

CAT#: CF813500

Carrier-free (BSA/glycerol-free) MICB mouse monoclonal antibody, clone OTI4E9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICB/MICA (RC222315/RC204447, Middle/Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICB.(1:1000)

Specifications

Product Data

• Clone Name: OTI4E9
• Applications: WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MICB (NP_005922) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.4 kDa
• Gene Name: Homo sapiens MHC class I polypeptide-related sequence B (MICB), transcript variant 1, mRNA.
• Database Link:
  NP_005922
  Entrez Gene 4277 Human
  UniProt ID Q29980
• Background: This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
• Synonyms: PERB11.2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Natural killer cell mediated cytotoxicity