MAP2 Mouse Monoclonal Antibody [Clone ID: OTI5F8]

CAT#: CF813551

Carrier-free (BSA/glycerol-free) MAP2 mouse monoclonal antibody, clone OTI5F8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2 (RC216775, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2.(1:1000)

Specifications

Product Data

• Clone Name: OTI5F8
• Applications: WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1507-1827 of human MAP2 (NP_002365) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 199.3 kDa
• Gene Name: Homo sapiens microtubule associated protein 2 (MAP2), transcript variant 1, mRNA.
• Database Link:
  NP_002365
  Entrez Gene 17756 MouseEntrez Gene 4133 Human
  UniProt ID P11137
• Background: This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010].
• Synonyms: MAP-2; MAP2A; MAP2B; MAP2C

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS