CD133 (PROM1) Mouse Monoclonal Antibody [Clone ID: OTI4A8]

CAT#: CF813574

Carrier-free (BSA/glycerol-free) PROM1 mouse monoclonal antibody, clone OTI4A8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROM1 (RC221611, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROM1.(1:1000)

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  Western blot analysis of extracts (35ug) from 4 cell lines lysates by using anti-PROM1 monoclonal antibody. (1:1000)

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  Flow cytometric analysis of living 293T cells transfected with PROM1 overexpression plasmid (RC221611), Red)/empty vector (PS100001, Blue) using anti-PROM1 antibody (TA813574). Cells incubated with a non-specific antibody (Green) were used as isotype control.（1：100）

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  Flow cytometric analysis of living HT29 cells, using anti-PROM1 antibody(TA813574, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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  Flow cytometric analysis of living HCT116 cells, using anti-PROM1 antibody(TA813574, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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  Flow cytometric analysis of living NCCIT cells, using anti-PROM1 antibody(TA813574, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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Specifications

Product Data

• Clone Name: OTI4A8
• Applications: FC, WB
• Recommended Dilution: WB 1:1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PROM1 (NP_006008) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 97 kDa
• Gene Name: Homo sapiens prominin 1 (PROM1), transcript variant 1, mRNA.
• Database Link:
  NP_006008
  Entrez Gene 8842 Human
  UniProt ID O43490
• Background: This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009].
• Synonyms: AC133; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane