MelanA (MLANA) Mouse Monoclonal Antibody [Clone ID: OTI1A2]

CAT#: CF813599

Carrier-free (BSA/glycerol-free) MLANA mouse monoclonal antibody, clone OTI1A2

Product Images

Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-MLANA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min, Cat# TA813599)

Specifications

Product Data

• Clone Name: OTI1A2
• Applications: IHC
• Recommended Dilution: IHC 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 48-118 of human MLANA (NP_005502) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13.2 kDa
• Gene Name: Homo sapiens melan-A (MLANA), mRNA.
• Database Link:
  NP_005502
  Entrez Gene 2315 Human
  UniProt ID Q16655
• Background: Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. [UniProtKB/Swiss-Prot Function]
• Synonyms: MART-1; MART1

Reference Data

• Protein Families: Transmembrane