TNFRSF14 Mouse Monoclonal Antibody [Clone ID: OTI5C5]

CAT#: CF813622

Carrier-free (BSA/glycerol-free) TNFRSF14 mouse monoclonal antibody, clone OTI5C5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF14 (RC201167, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF14.(1:1000)

• 

  Flow cytometric analysis of living 293T cells transfected with TNFRSF14 overexpression plasmid (RC201167), Red)/empty vector (PS100001, Blue) using anti-TNFRSF14 antibody (TA813622). Cells incubated with a non-specific antibody (Green) were used as isotype control.（1：100）

Specifications

Product Data

• Clone Name: OTI5C5
• Applications: FC, WB
• Recommended Dilution: WB 1:1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TNFRSF14 (NP_003811) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.4 kDa
• Gene Name: Homo sapiens TNF receptor superfamily member 14 (TNFRSF14), transcript variant 1, mRNA.
• Database Link:
  NP_003811
  Entrez Gene 8764 Human
  UniProt ID Q92956
• Background: This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014].
• Synonyms: ATAR; CD270; HVEA; HVEM; LIGHTR; TR2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction