SLAMF7 Mouse Monoclonal Antibody [Clone ID: OTI3B6]

CAT#: CF813680

Carrier-free (BSA/glycerol-free) SLAMF7 mouse monoclonal antibody, clone OTI3B6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLAMF7 (RC220985, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLAMF7.(1:1000)

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-SLAMF7 monoclonal antibody. (1:500)

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  Flow cytometric analysis of living 293T cells transfected with SLAMF7 overexpression plasmid (RC220985), Red)/empty vector (PS100001, Blue) using anti-SLAMF7 antibody (TA813680). Cells incubated with a non-specific antibody (Green) were used as isotype control.(1:100)

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  Flow cytometric analysis of living OPM2 cells, using anti-SLAMF7 antibody(TA813680, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

Specifications

Product Data

• Clone Name: OTI3B6
• Applications: FC, WB
• Recommended Dilution: WB 1:500-1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SLAMF7 (NP_067004) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.2 kDa
• Gene Name: Homo sapiens SLAM family member 7 (SLAMF7), transcript variant 1, mRNA.
• Database Link:
  NP_067004
  Entrez Gene 57823 Human
  UniProt ID Q9NQ25
• Background: Isoform 3 does not mediate any NK cell activation. [UniProtKB/Swiss-Prot Function]
• Synonyms: 19A; CD319; CRACC; CS1

Reference Data

• Protein Families: Druggable Genome, Transmembrane