CD28 Mouse Monoclonal Antibody [Clone ID: OTI9G10]

CAT#: CF813687

Carrier-free (BSA/glycerol-free) CD28 mouse monoclonal antibody, clone OTI9G10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD28 (RC211318, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD28.(1:500)

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  Flow cytometric analysis of living 293T cells transfected with CD28 overexpression plasmid (RC211318), Red)/empty vector (PS100001, Blue) using anti-CD28 antibody (TA813687). Cells incubated with a non-specific antibody (Green) were used as isotype control.(1:100)

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  Flow cytometric analysis of living Jurkat cells, using anti-CD28 antibody(TA813687, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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  Flow cytometric analysis of living RPMI-8226 cells, using anti-CD28 antibody(TA813687, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

Specifications

Product Data

• Clone Name: OTI9G10
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD28 (NP_006130) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.1 kDa
• Gene Name: Homo sapiens CD28 molecule (CD28), transcript variant 1, mRNA.
• Database Link:
  NP_006130
  Entrez Gene 940 Human
  UniProt ID P10747
• Background: The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
• Synonyms: Tp44

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, T cell receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis