MLH1 Mouse Monoclonal Antibody [Clone ID: OTI4H4]

CAT#: CF813718

Carrier-free (BSA/glycerol-free) MLH1 mouse monoclonal antibody, clone OTI4H4

Product Images

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3 min, TA813718)

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  Immunohistochemical staining of paraffin-embedded Human Colon cancer tissues with expression (left) and loss (right) of MLH1 protein using anti-MLH1 mouse monoclonal antibody (TA813718). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3 min.

Specifications

Product Data

• Clone Name: OTI4H4
• Applications: IHC
• Recommended Dilution: IHC 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 339-558 of human MLH1 (NP_000240) produced in E.coli.
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.6 kDa
• Gene Name: mutL homolog 1
• Database Link:
  NP_000240
  Entrez Gene 17350 MouseEntrez Gene 81685 RatEntrez Gene 4292 Human
  UniProt ID P40692
• Background: The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]
• Synonyms: COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MMRCS1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer