NSE (ENO2) Mouse Monoclonal Antibody [Clone ID: OTI6E11]

CAT#: CF813804

Carrier-free (BSA/glycerol-free) NSE mouse monoclonal antibody, clone OTI6E11

Product Images

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NSE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA813804)

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-NSE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min, Cat# TA813804)

Specifications

Product Data

• Clone Name: OTI6E11
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 188-293 of human NSE (NP_001966) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.3 kDa
• Gene Name: Homo sapiens enolase 2 (ENO2), mRNA.
• Database Link:
  NP_001966
  Entrez Gene 13807 MouseEntrez Gene 24334 RatEntrez Gene 2026 Human
  UniProt ID P09104
• Background: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
• Synonyms: HEL-S-279; NSE

Reference Data

• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation