HSD17B13 Mouse Monoclonal Antibody [Clone ID: OTI5C4]

CAT#: CF814158

Carrier-free (BSA/glycerol-free) mouse monoclonal HSD17B13 Antibody,Clone OTI5C4

Product Images

Western blot analysis of overexpressed human HSD17B13 lysate (5ug LY405941, LEFT) and purified recombinant protein (0.02ug TP313132, RIGHT) by using anti-HSD17B13 antibody (TA814158).

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  HEK293T cells were transfected with 10 different plasmids of human HSD cDNA ( HSD11B1, HSD17B2, HSD17B3, HSD17B4, HSD17B6, HSD17B7, HSD17B8, HSD17B10, HSD17B11, HSD17B13) for 48 hrs and lysed. Equivalent amounts of cell lysates (10ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B13 mouse monoclonal antibody. HSD17B13 was positive, while all others were negative. (TA814158, 1：500)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSD17B13 mouse monoclonal antibody, TA814158 (1:200). *Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HSD17B13 mouse monoclonal antibody, TA814158 (1:200). *Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min.

Specifications

Product Data

• Clone Name: OTI5C4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500-1:1000, IHC 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 20-300 of human HSD17B13 produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.7 kDa
• Gene Name: Homo sapiens hydroxysteroid 17-beta dehydrogenase 13 (HSD17B13), transcript variant A, mRNA.
• Database Link:
  NP_835236
  Entrez Gene 345275 Human
  UniProt ID Q7Z5P4
• Synonyms: HMFN0376; NIIL497; SCDR9; SDR16C3

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane