Mesothelin (MSLN) Mouse Monoclonal Antibody [Clone ID: OTI7C2]

CAT#: CF814168

Carrier-free (BSA/glycerol-free) MSLN mouse monoclonal antibody, clone OTI7C2

Product Images

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MSLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814168)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MSLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA814168)

Specifications

Product Data

• Clone Name: OTI7C2
• Applications: IHC
• Recommended Dilution: IHC 1:1500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MSLN (NP_037536) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.99 kDa
• Gene Name: mesothelin
• Database Link:
  NP_037536
  Entrez Gene 10232 Human
  UniProt ID Q13421
• Background: This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
• Synonyms: MPF; SMRP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane