P Glycoprotein (ABCB1) Mouse Monoclonal Antibody [Clone ID: OTI2A2]

CAT#: CF814175

Carrier-free (BSA/glycerol-free) MDR-1/ABCB1 mouse monoclonal antibody, clone OTI2A2

Product Images

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MDR-1/ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA814175)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MDR-1/ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA814175)

Specifications

Product Data

• Clone Name: OTI2A2
• Applications: IHC
• Recommended Dilution: IHC 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MDR-1/ABCB1 (NP_000918) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 141.3 kDa
• Gene Name: Homo sapiens ATP binding cassette subfamily B member 1 (ABCB1), transcript variant 3, mRNA.
• Database Link:
  NP_000918
  Entrez Gene 170913 RatEntrez Gene 5243 Human
  UniProt ID P08183
• Background: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Feb 2017]
• Synonyms: ABC20; CD243; CLCS; GP170; MDR1; P-GP; PGY1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: ABC transporters