Perforin (PRF1) Mouse Monoclonal Antibody [Clone ID: OTI10A2]

CAT#: CF814203

Carrier-free (BSA/glycerol-free) Perforin-1 mouse monoclonal antibody, clone OTI10A2

Product Images

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-Perforin-1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3 min, Cat# TA814203)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-Perforin-1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3 min, Cat# TA814203)

Specifications

Product Data

• Clone Name: OTI10A2
• Applications: IHC
• Recommended Dilution: IHC 1:75
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 351-555 of human Perforin-1 (NP_005032) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.38 kDa
• Gene Name: Homo sapiens perforin 1 (PRF1), transcript variant 1, mRNA.
• Database Link:
  NP_005032
  Entrez Gene 5551 Human
  UniProt ID P14222
• Background: This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]
• Synonyms: HPLH2; P1; PFP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis