Desmin (DES) Mouse Monoclonal Antibody [Clone ID: OTI3B12]

CAT#: CF814318

Carrier-free (BSA/glycerol-free) Desmin mouse monoclonal antibody, clone OTI3B12

Product Images

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-Desmin mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA814318)

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  Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-Desmin mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, Cat# TA814318)

Specifications

Product Data

• Clone Name: OTI3B12
• Applications: IHC
• Recommended Dilution: IHC 1:1500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Desmin (NP_001918). The exact sequence is proprietary.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: Homo sapiens desmin (DES), mRNA.
• Database Link:
  NP_001918
  Entrez Gene 13346 MouseEntrez Gene 64362 RatEntrez Gene 1674 Human
  UniProt ID P17661
• Background: This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]
• Synonyms: CDCD3; CMD1F; CSM1; CSM2; LGMD1D; LGMD1E; LGMD2R

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)