PAH Mouse Monoclonal Antibody [Clone ID: OTI4F6]

CAT#: CF815031

Carrier-free (BSA/glycerol-free) PAH mouse monoclonal antibody,clone OTI4F6

Product Images

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1), human PAH plasmid (RC204694, lane 2), mouse PAH plasmid (MR207240, lane 3), using anti-PAH antibody TA815031 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Western blot analysis of extracts(50ug) from HepG2 cell lines lysates by using anti-PAH monoclonal antibody. (TA815031, 1:500)

Specifications

Product Data

• Clone Name: OTI4F6
• Applications: WB
• Recommended Dilution: WB1:500
• Reactivities: Human, Mouse
• Modifications: Modification
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PAH (NP_000268) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.8 kDa
• Gene Name: Homo sapiens phenylalanine hydroxylase (PAH), transcript variant 1, mRNA.
• Database Link:
  NP_000268
  Entrez Gene 18478 MouseEntrez Gene 5053 Human
  UniProt ID P00439
• Background: This gene encodes a member of the biopterin-dependent aromatic amino acid hydroxylase protein family. The encoded phenylalanine hydroxylase enzyme hydroxylates phenylalanine to tyrosine and is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. [provided by RefSeq, Aug 2017]
• Synonyms: PH; PKU; PKU1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism