ADAR1 (ADAR) Mouse Monoclonal Antibody [Clone ID: OTI5G2]

CAT#: CF815219

Carrier-free (BSA/glycerol-free) ADAR mouse monoclonal antibody,clone OTI5G2

Product Images

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human ADAR plasmid (RC219761, lane 2), mouse ADAR plasmid (MR211714, lane 3) using anti-ADAR antibody TA815219 (1:2000). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Western blot analysis of extracts (50ug per lane) from 3 cell lines lysates by using anti-ADAR monoclonal antibody(TA815219, 1:1000)

Specifications

Product Data

• Clone Name: OTI5G2
• Applications: WB
• Recommended Dilution: WB1:1000-1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment of human ADAR (NP_001020278) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 103.6 kDa
• Gene Name: Homo sapiens adenosine deaminase RNA specific (ADAR), transcript variant 4, mRNA.
• Database Link:
  NP_001020278
  Entrez Gene 103 Human
  UniProt ID P55265
• Background: This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
• Synonyms: ADAR1; AGS6; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88DSRBP; P136

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cytosolic DNA-sensing pathway