AAV5 Mouse Monoclonal Antibody [Clone ID: OTI16H1]

CAT#: CF816057

Carrier-free (BSA/glycerol-free)AAV5 mouse monoclonal antibody,clone OTI16H1

Specifications

Product Data

• Clone Name: OTI16H1
• Applications: WB
• Recommended Dilution: WB1:1000
• Reactivities: Adenovirus 5
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: AAV5 capsids
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 100KD
• Background: AAV vectors are promising delivery tools for human gene therapy. AAV is a single-stranded DNA parvovirus with a 4.7 kb genome composed of the rep and cap genes flanked by inverted terminal repeats (ITRs). The rep gene encodes non-structural proteins involved in viral replication, packaging, and genomic integration, whereas the cap gene codes for structural proteins (VP1, VP2, VP3) that assemble to form the viral capsid, which serves as the viral gene delivery vehicle.